Background: As an amateur grower, growing fungi for culinary use, I am frequently encountering contamination issues, most often from a rust colored contaminant, but others as well in spite of using a still air box. Given my current amateur status, it seems like a waste of resources to invest in a flow hood. Selective media with readily available supplies, if possible, would solve this issue until investments in more advanced equipment is made.
Proof of Concept:
- Four glad mini rounds were filled with agar and sterilized, 2 with an antibacterial agar(1), 2 with an anti-fungal agar(2).
- One of each were inoculated with spores and one of each with mycelium.
Containers were placed in incubator @ 85 degrees.
- Both anti fungal media show no signs of growth or contamination.
- Both anti bacterial media show signs of desired fungal growth, but also have rust colored contaminant.
- Anti bacterial recipe seems to work. Fine tuning with a more controllable recipe should be explored.
- Anti fungal recipe seems to kill desired mycelium and prevent desired spores from maturing. Perhaps less anti-fungal, or an anti fungal that uses a different mechanism might be able to select for desired fungi and against fungal contaminants.
- Anti Bacterial
- 1.25 mL CVS triple ointment (400 units Bacitracin Zinc, 5000 Polymyxin, & 3.5 mg neomycin per 14g tube.
- 1/4 cup Potato flake agar
- Ointment was added to hot agar and mixed thoroughly, then divided between two mini round containers.
- Anti fungal
- 1 mL 2% miconazole nitrate liquid
- 1/4 cup potato flake agar
- Made similarly to antibacterial media.